Part:BBa_J435381

T2T3_fuGFP

Free-Use GFP. Note: Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.

FLANKED 5' fusion site: A, 3' fusion site: GCTT

Source organism: , Target organism: Escherichia coli

Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003423.html

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 682
  Illegal AgeI site found at 151
  
• 1000
  COMPATIBLE WITH RFC[1000]